Microscope, Cells & Organelles
Part I
Table 1. Microscope
magnification.
Lens mag.
Ocular mag. (fill
in) Total mag. (fill in)
Scanning 4x
multiplied by 10 x = 40 x
Low power 10x
multiplied by 10 x
= 100 x
High power 40x multiplied
by 10 x
=
400 x
Oil immersion 100x
multiplied by 10 x = 1000 x
Part II
Cheek Smear Slide
- Nucleus
- Cytoplasm
- Plasm Membrane
Onion Root Slide
What structure separates the contents of the nucleus from
the cytoplasm?
The Nuclear Membrane separates the contents of the nucleus
from the cytoplasm.
What is the function of the nucleoli? Why is this
important?
The nucleoli make ribosomal subunits from proteins &
ribosomal RNA. The importance of the ribosomal is that it makes
proteins for the cell.
Other than the nucleoplasm and the nucleoli, what are
three other materials found in a nucleus?
Other materials found in a nucleus are: A)DNA, B) Chromatin, C) Nuclear Pore
Which objective should be in place when you start?
Objective 4X should be your starting point when looking
through the oculars.
Which focusing knob do you first use when you begin
looking at a slide?
The Coarse Adjusting knob will be used to help
you focus.
What do you adjust if you can see two overlapping circles
with part of the object in each circle?
While looking through eyepiece (Oculars) you adjust them in
or out till you only see one circle.
How do you move the slide on the stage?
To move the slide on the stage you would use the coarse adjustment
knob.
What two devices can maneuvered to increase or decrease
light intensity?
The two diveces that can increase or decrease light are Diaphragm/light
intensity knob and the condenser knob.
What is the name of the highest magnifying objective?
The highest magnifying objective is 10X or High Dry
Which objective allows you to see the largest area of the
object that you are viewing?
4X would be the best objective to use in this circumstances.
Part
III
Record your predictions and rationale. The
Carrot immersed in salt water will shrink and be limp due to dehydration (the
saltwater solution drawing the water out of the carrot) causing the string to
fall off and the carrot in freshwater will expand due to adding hydration
causing the string to tighten and possibly extend.
Note if there are any differences in the tightness of the strings and the textures
for both carrots:
The carrot in the freshwater expanded causing the string to tightened. Where as the saltwater carrot became limp and the string was loose.
The carrot in the freshwater expanded causing the string to tightened. Where as the saltwater carrot became limp and the string was loose.
Observe which type of water caused an increase in cell
size. The carrot in the
freshwater expanded causing the string to tighten. Whereas
the saltwater carrot became limp and the string fell off.
Observe which type of water caused a decrease in
cell size. The
Saltwater caused a decrease in cell size and the Freshwater caused an increase
in cell size.
How did the actual results in the measurements compared to
your predictions? My prediction was
pretty much on task. The only difference
I noticed was the saltwater carrot started to float approximately after 23
hours.
What is moving? The Water is moving. Why? The water is trying to
find a equal Librium in concentration.
How is this important for
the health of cells? Health Cells depend on
water to keep an even balance of Osmotic pressure. If a person were
to drink a larger amount of saltwater there body would start to dehydrate.
Start
Completion
Table 2. Starting and Ending Measurments:
|
Length at Start of24Hours
|
Length at End of24Hours
|
Width at Start of 24Hours
|
Width at End of 24Hours
|
|
|
Freshwater Carrot
|
3”
|
3 ¼”
|
1 ½”
|
1 ¼”
|
|
Saltwater Carrot
|
3”
|
2 ½”
|
1 ½”
|
1 ¼”
|
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